Necrotizing meningoencephalitis in atypical dog breeds: a case series and literature review.

BackgroundCanine necrotizing meningoencephalitis (NME) is a fatal, noninfectious inflammatory disease of unknown etiology. NME has been reported only in a small number of dog breeds, which has led to the presumption that it is a breed-restricted disorder.Hypothesis/objectivesOur objective was to describe histopathologically confirmed NME in dog breeds in which the condition has not been reported previously and to provide preliminary evidence that NME affects a wider spectrum of dog breeds than previously reported.AnimalsFour dogs with NME.MethodsArchives from 3 institutions and from 1 author's (BS) collection were reviewed to identify histopathologically confirmed cases of NME in breeds in which the disease has not been reported previously. Age, sex, breed, survival from onset of clinical signs, and histopathologic findings were evaluated.ResultsNecrotizing meningoencephalitis was identified in 4 small dog breeds (Papillon, Shih Tzu, Coton de Tulear, and Brussels Griffon). Median age at clinical evaluation was 2.5 years. Histopathologic abnormalities included 2 or more of the following: lymphoplasmacytic or histiocytic meningoencephalitis or encephalitis, moderate-to-severe cerebrocortical necrosis, variable involvement of other anatomic locations within the brain (cerebellum, brainstem), and absence of detectable infectious agents.Conclusions and clinical importanceUntil now, NME has only been described in 5 small dog breeds. We document an additional 4 small breeds previously not shown to develop NME. Our cases further illustrate that NME is not a breed-restricted disorder and should be considered in the differential diagnosis for dogs with signalment and clinical signs consistent with inflammatory brain disease

Facile: a command-line network compiler for systems biology

<p>Abstract</p> <p>Background</p> <p>A goal of systems biology is the quantitative modelling of biochemical networks. Yet for many biochemical systems, parameter values and even the existence of interactions between some chemical species are unknown. It is therefore important to be able to easily investigate the effects of adding or removing reactions and to easily perform a bifurcation analysis, which shows the qualitative dynamics of a model for a range of parameter values.</p> <p>Results</p> <p>We present Facile, a Perl command-line tool for analysing the dynamics of a systems biology model. Facile implements the law of mass action to automatically compile a biochemical network (written as, for example, <monospace>E + S <-> C</monospace>) into scripts for analytical analysis (Mathematica and Maple), for simulation (XPP and Matlab), and for bifurcation analysis (AUTO). Facile automatically identifies mass conservations and generates the reduced form of a model with the minimum number of independent variables. This form is essential for bifurcation analysis, and Facile produces a C version of the reduced model for AUTO.</p> <p>Conclusion</p> <p>Facile is a simple, yet powerful, tool that greatly accelerates analysis of the dynamics of a biochemical network. By acting at the command-line and because of its intuitive, text-based input, Facile is quick to learn and can be incorporated into larger programs or into automated tasks.</p

High prevalence of scrapie in a dairy goat herd: tissue distribution of disease-associated PrP and effect of PRNP genotype and age

Following a severe outbreak of clinical scrapie in 2006–2007, a large dairy goat herd was culled and 200 animals were selected for post-mortem examinations in order to ascertain the prevalence of infection, the effect of age, breed and PRNP genotype on the susceptibility to scrapie, the tissue distribution of diseaseassociated PrP (PrP$^{\rm d}$), and the comparative efficiency of different diagnostic methods. As determined by immunohistochemical (IHC) examinations with Bar224 PrP antibody, the prevalence of preclinical infection was very high (72/200; 36.0%), with most infected animals being positive for PrP$^{\rm d}$ in lymphoreticular system (LRS) tissues (68/72; 94.4%) compared to those that were positive in brain samples (38/72; 52.8%). The retropharyngeal lymph node and the palatine tonsil showed the highest frequency of PrP$^{\rm d}$ accumulation (87.3% and 84.5%, respectively), while the recto-anal mucosa-associated lymphoid tissue (RAMALT) was positive in only 30 (41.7%) of the infected goats. However, the efficiency of rectal and palatine tonsil biopsies taken shortly before necropsy was similar. The probability of brain and RAMALT being positive directly correlated with the spread of PrP$^{\rm d}$ within the LRS. The prevalence of infection was influenced by PRNP genetics at codon 142 and by the age of the goats: methionine carriers older than 60 months showed a much lower prevalence of infection (12/78; 15.4%) than those younger than 60 months (20/42; 47.6%); these last showed prevalence values similar to isoleucine homozygotes of any age (40/80; 50.0%). Two of seven goats with definite signs of scrapie were negative for PrP$^{\rm d}$ in brain but positive in LRS tissues, and one goat showed biochemical and IHC features of PrP$^{\rm d}$ different from all other infected goats. The results of this study have implications for surveillance and control policies for scrapie in goats

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OPTIMALISASI MITIGASI BAHAYA GEMPA BUMI MELALUI PENYULUHAN RUMAH TAHAN GEMPA DI KELURAHAN REWARANGGA SELATAN PROVINSI NUSA TENGGARA TIMUR

Abstrak: Permasalahan utama mitra adalah adanya bangunan rumah tinggal yang rentan terhadap bahaya gempa bumi, dan belum adanya pengetahuan yang baik tentang tatacara membangun rumah tahan gempa sesuai standar yang berlaku. Tujuan kegiatan Pengabdian Masyarakat (PkM) ini untuk mencegah bencana akibat gempa melalui peningkatkan pengetahuan dan keterampilan masyarakat khususnya para tukang bangunan tentang rumah tahan gempa. Mitra kegiatan ini adalah Lurah Kelurahan Rewarangga Selatan dan kelompok sasarannya adalah masyarakat khususnya para tukang bangunan di RT 02 RW 01 Kelurahan Rewarangga Selatan. Peserta pelatihan dan bimbingan teknis berjumlah 30 orang, dan metode yang digunakan adalah penyuluhan dan pelatihan. Hasil evaluasi menunjukkan adanya peningatan sebesar 16% terhadap kemampuan dan keterampilan peserta yang diperoleh dengan membandingkan hasil test awal dan akhir, sedangkan evaluasi kepuasan mitra terhadap pelaksanaan PkM menunjukkan 80% menyatakan sangat puas, dengan demikian PkM yang dilaksanakan berhasil meningkatkan pengetahuan dan keterampilan masyarakat tentang mitigasi bencana gempa melalui bangunan rumah tinggal tahan gempa.Abstract: The partners' main problem is the existence of residential buildings that are vulnerable to earthquake hazards, and the lack of good knowledge about procedures for building earthquake-resistant houses according to applicable standards. The purpose of this Community Service (PkM) activity is to prevent earthquake-induced disasters by increasing the knowledge and skills of the community, especially the builders about earthquake-resistant houses. The partner of this activity is the Head of the South Rewarangga Sub-district and the target group is the community, especially the builders in RT 02 RW 01, South Rewarangga Sub-district. The participants of the training and technical guidance are 30 people, and the method used is counseling and training. The results of the evaluation showed an increase of 16% in the abilities and skills of the participants obtained by comparing the results of the initial and final tests, while the evaluation of partner satisfaction with the implementation of the PkM showed 80% stated that they were very satisfied, thus the PkM implemented succeeded in increasing the knowledge and skills of the community about mitigation. Earthquake disasters through earthquake-resistant residential buildings

Pathogenesis of scrapie in ARQ/ARQ sheep after subcutaneous infection: effect of lymphadenectomy and immune cell subset changes in relation to prion protein accumulation.

Although it is well established that the infectious agent can replicate in the lymphoreticular system (LRS) early after inoculation, the information on pathways or cells involved in the dissemination of scrapie from the point of inoculation is limited. In order to gain a better understanding on these mechanisms 16 ARQ/ARQ, polymorphic or non polymorphic Suffolk or Romney lambs were inoculated subcutaneously with a Suffolk scrapie brain homogenate in the drainage area of the prefemoral lymph node. Fourteen lambs were then either subjected to early or late surgical removal of the prefemoral lymph nodes or not subjected to lymphadectomy and used as positive controls. Eleven animals were culled at a preclinical stage of the disease, and only 5, including 2 positive controls, were killed after reaching clinical end point. Of 5 polymorphic animals killed at preclinical stages of infection, two did not show any evidence of infection, two showed little involvement of LRS tissues and little or none in brain, and one showed widespread LRS involvement but mild PrPd accumulation in the CNS. This was in contrast with the findings in non-polymorphic sheep which, at comparable dpi, showed a complete attack rate with widespread PrPd accumulation in LRS tissues and many of them also in the CNS. The only polymorphic sheep left to develop clinical signs reached enpoint with a more protracted incubation period than the non-polymorphic sheep, but with similar PrPd magnitudes in the LRS or brain. The only change that appears to be related to PrPd accumulation in the LNs is the increase in CD21+ cells indistinctly in polymorphic or polymorphic animals

Objetivos de Desarrollo Sostenible y Aprendizaje Servicio en la docencia universitaria: un estudio de caso en el área de Biblioteconomía y Documentación

Las universidades se configuran como instituciones clave para responder activamente al cumplimiento de los Objetivos de Desarrollo Sostenible (ODS) de la “Agenda 2030” de la ONU. A pesar de que han surgido algunas iniciativas para responder a dicho reto, se constata la necesidad deincrementar las acciones que respondan a tal finalidad yque emerjandesde el ámbito universitario. A dicho enfoque se vincula el estudio de caso que se presenta. Se ha desarrollado una expe-riencia de docencia universitaria en el área de Bibliote-conomía y Documentación, sustentada en establecer una vinculación entre la metodología docente de Aprendizaje Servicio (ApS) y la incorporaciónde una perspectiva basada en los ODS en la formación de los futuros bibliotecarios, archiveros y documentalistas. Universities are key institutions to respond to the achievement of the Sustainable Development Goals (SDGs) of the UN "2030 Agenda". Although some initiatives have emerged to respond to this challenge, more university actions and initiatives have to be taken. This research is a case study based on the approach mentioned. A teaching experience at university level has been developed in the Library and Information Science area to link the Service Learning (SL) methodology with an innovative teaching approach focused on the SDGs to improve librarians, archivists and information professionals training

All clinically-relevant blood components transmit prion disease following a single blood transfusion: a sheep model of vCJD

Variant CJD (vCJD) is an incurable, infectious human disease, likely arising from the consumption of BSE-contaminated meat products. Whilst the epidemic appears to be waning, there is much concern that vCJD infection may be perpetuated in humans by the transfusion of contaminated blood products. Since 2004, several cases of transfusion-associated vCJD transmission have been reported and linked to blood collected from pre-clinically affected donors. Using an animal model in which the disease manifested resembles that of humans affected with vCJD, we examined which blood components used in human medicine are likely to pose the greatest risk of transmitting vCJD via transfusion. We collected two full units of blood from BSE-infected donor animals during the pre-clinical phase of infection. Using methods employed by transfusion services we prepared red cell concentrates, plasma and platelets units (including leucoreduced equivalents). Following transfusion, we showed that all components contain sufficient levels of infectivity to cause disease following only a single transfusion and also that leucoreduction did not prevent disease transmission. These data suggest that all blood components are vectors for prion disease transmission, and highlight the importance of multiple control measures to minimise the risk of human to human transmission of vCJD by blood transfusion

A renewed rise in global HCFC-141b emissions between 2017???2021

Global emissions of the ozone-depleting gas HCFC-141b (1,1-dichloro-1-fluoroethane, CH3CCl2F) derived from measurements of atmospheric mole fractions increased between 2017 and 2021 despite a fall in reported production and consumption of HCFC-141b for dispersive uses. HCFC-141b is a controlled substance under the Montreal Protocol, and its phase-out is currently underway, after a peak in reported consumption and production in developing (Article 5) countries in 2013. If reported production and consumption are correct, our study suggests that the 2017–2021 rise is due to an increase in emissions from the bank when appliances containing HCFC-141b reach the end of their life, or from production of HCFC-141b not reported for dispersive uses. Regional emissions have been estimated between 2017–2020 for all regions where measurements have sufficient sensitivity to emissions. This includes the regions of northwestern Europe, east Asia, the United States and Australia, where emissions decreased by a total of 2.3 ± 4.6 Gg yr−1, compared to a mean global increase of 3.0 ± 1.2 Gg yr−1 over the same period. Collectively these regions only account for around 30 % of global emissions in 2020. We are not able to pinpoint the source regions or specific activities responsible for the recent global emission rise